To study how viruses replicate inside cells, we have constructed a light-sheet microscope in the lab. Below are some movies from our recent paper (Bhagwat et al Nature Communications 2021), which describes how influenza viruses and other respiratory viruses hijack the movement host endosomes contains the cellular GTPase Rab11A.
Visualization of influenza viral RNA transport in MDCK cell using WSN PA-eGFP virus. Taken on iSPIM, developed by Dr. Hari Shroff (NIBIB), 2 seconds per cell volume with continuous imaging for 30 min at 16 hours post infection. Published in Lakdawala et al PLoS Pathogens 2011.
Spread of influenza viral infection using WSN PA-eGFP virus in MDCK cells using confocal microscopy for 16 hours, one volume every 10 min.